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Data Analysis
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2. Imprints of the Wound
For a better quantitative assessment of the bacterial load, we take imprints
of the wound surface fluid using nitrocellulosis filters (impregnated with a
culture medium).
The filter pad technique ensures not only a better quantitative estimate of
the bacterial contamination of the wound but enables the characterization
of its spatial distribution.
Microbiological experiments have shown that the ''filter pad'' method is more
reproducibie than the usual ''smear technique'' and yields a higher sensitivity
to bacterial contamination of the wound.
We have developed a method to segment area covered by bacteria on filter pads
based on symbolic representations, which allows us to calculate the change in
bacterial load after consecutive treatments. Our results indicate that plasma
treatment induces a germ reduction higher than 25% with respect to that of the
control group (wounds receiving only standard antibacterial treatment). These
results are in agreement with those obtained by counting bacterial colonies
through visual inspection.
Read more:
- Chronic Wounds
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